FA0135 - PCR approach for quantitation of horse DNA
the aim of this project was to develop a real-time PCR approach for the quantitation of horse DNA using best measurement practice guidelines in the field of PCR and meat speciation. Additionally, a review of expressing meat adulteration in terms of w/w tissue mass and DNA:DNA copy numbers was conducted, in order to establish if guidance could be provided on the equivalence of these expression units.
The project showed that the real-time PCR assay developed in the study:
- Can accurately quantify the relative amount of horse DNA in a mixed sample, and exhibits good comparability to w/w gravimetric materials when samples and calibrants contain similar materials
- Is subject to the same limitations inherent in all meat quantitation approaches available: that of
converting DNA:DNA copy number measurements into w/w tissue measurements - Has significantly added value to the state-of-the-art of the science and made publically available an
approach for the quantitation of horse DNA in line with best measurement practice in the field - Is adaptable and “future-proofed” for its potential to incorporate and fully characterise the threshold for labelling, as proposed by the recently published EU guidance (EURL-AP, 2013)
File Name: | Final_report_FA0135.pdf |
File Size: | 856.98 KB |
File Type: | application/pdf |
Created Date: | 08-11-2015 |
Last Updated Date: | 08-11-2015 |