Heart marker protein - Extraction and ELISA

Full title

Standard Operating Procedure for the extraction of a heart marker protein in raw and processed meat products, and its subsequent detection and quantification by an ELISA


To perform ELISA assays on extracts of meat products using anti-cardiac troponin I (cTnI antibodies, in order to detect and quantify added bovine/ovine/porcine heart. The SOP can be used to analyse raw and cooked meat products.


Prepared by Lyndsey Durose, Nottingham Trent University Date July 2012


DEFRA SOP, Project FA0106

Publication version

Version 1.0, July 2012

Approval status

Approved by Authenticity Methods Working Group Date July 2012


Contains public sector information licensed under the Open Government Licence v3.0.

File Name: FA0106_14317_ELISAheartSOP_v1-0.pdf
File Size: 146.58 KB
File Type: application/pdf
Created Date: 08-11-2018
Last Updated Date: 08-11-2018