Offal marker proteins - extraction and semi-quantification
Description
Standard operating procedure for the extraction of offal marker proteins in raw and processed meat products, and their subsequent detection and semi-quantification
Scope
To probe Western blots of extracts of meat products with anti-heart specific protein 2 (H-SP2), anti-liver specific protein 3 (L-SP3), anti-lung specific protein 2 (Lu-SP2) and anti-kidney marker protein 1 (K-MP1) antibodies in order to detect and semi-quantify added bovine/ovine/porcine heart, liver, lung and kidney, respectively. K-MP1 is a protein expressed at high levels in kidney, small intestine and placenta and at lower levels in liver, pancreas, prostate, spleen, stomach and large intestine, however, it is not expressed in skeletal muscle or heart. The SOP can be used to analyse raw and cooked meat products.
Origin
DEFRA SOP project FA0122
Version
SOP FA0122(3) Version 1.2, January 2012
Author(s)
Prepared by Lyndsey Durose, Nottingham Trent University Date January 2016
Approval status
Reviewed by Authenticity Methods Working Group Date January 2016
Copyright
Contains public sector information licensed under the Open Government Licence v3.0.
File Name: | FA0122_14314_SOPFA0122_v1-2.pdf |
File Size: | 281.09 KB |
File Type: | application/pdf |
Created Date: | 08-11-2018 |
Last Updated Date: | 08-11-2018 |