- Abstract:
- PCR-RFLP analysis was shown to be capable of discriminating pure wild boar meat from pure pig meat but is not suitable for the analysis of more complex samples, such as meat from cross breeds, and mixtures of wild boar meat and pig meat. However, using microsatellite analysis, a panel of 8 microsatellite markers was identified that could distinguish pure wild boar meat from pure breed or cross-breed pig meat, and a panel of 21 microsatellites was capable of identifying mixtures containing 50% or greater wild boar DNA mixed with pig DNA as wild boar-pig mixtures. The method using these microsatellite markers was optimised, resulting in a standard operating procedure (SOP) for the verification of meat from wild boar, which is suitable for the analysis of cuts of meat. The SOP was internally validated, and was shown to correctly assign unknown samples of meat cuts as either wild boar or pig.
- PubYear:
- 2010
- Keywords:
- FundingBody:
- Food Standards Agency (UK)
- Contractor:
- Fera Science Ltd
- ProjCode:
- QO1129
- Associated:
- Type:
- Research: Laboratory Based
- OpenAccess:
- Yes
- URL:
- https://www.nal.usda.gov/research-tools/food-safety-research-projects/development-and-validation-dna-marker-methods
- Comments:
- NA