- Abstract:
- Defra project FA0172 (2019) investigated the use of Kompetitive Allele Specific PCR (KASP) assays to authenticate Basmati rice varieties and detect adulteration with non-Basmati rice. Existing authentication approaches using microsatellite DNA markers which cannot reliably distinguish all approved Basmati varieties. The study evaluated whether single nucleotide polymorphisms (SNPs) and insertion/deletion (InDel) markers could be developed into KASP assays capable of distinguishing Basmati from non-Basmati varieties. Results demonstrated that KASP assays provide a feasible and cost-effective method for differentiating Basmati and non-Basmati rice varieties, with potential to improve regulatory testing and industry verification of Basmati labelling.
- PubYear:
- 2019
- Keywords:
- Basmati rice authentication, Kompetitive Allele Specific PCR (KASP), DNA marker assays, SNP and InDel markers, Rice variety identification, Food authenticity testing, Molecular genotyping, Basmati Code of Practice
- FundingBody:
- Defra, UK
- Contractor:
- Bangor University
- ProjCode:
- FA0172
- Associated:
- Type:
- Research: Laboratory Based
- OpenAccess:
- Yes
- URL:
- #
- Comments: